कैसे टू यूज एन ऑप्टिकल माइक्रोस्कोप
1. ऑपरेशन कदम और सावधानियां
(1) ईमानदार माइक्रोस्कोप
1. Hold the mirror arm with your right hand and the mirror base with your left hand to keep the mirror body upright. The desktop should be clean and stable, and you should choose a place near a window or with sufficient light. The single tube is generally placed on the left side, 3 to 4 cm away from the edge of the table.
2. Clean and check the माइक्रोस्कोप /दोषपूर्ण और क्लीन. मैकेनिकल पार्ट्स of the mirror body can by wiped with a clean सॉफ्ट क्लॉथ. लेंस लेंस dep with lens cleaning paper. अगर there in gnue or गंदगी, clean it with a small मात्रा xylene.
3. बढ़ाएँ दर्पण दर्पण बैरल to a दूरी 1 to 2 cm से the चरण, और aline कम-आवर्धन दर्पण दर्पण दह प्रकाश छेद के साथ. समायोजन अपर्चर और परावर्तक, उपयोग flat mirror जब प्रकाश मजबूत, उपयोग अवतल दर्पण जब प्रकाश is कमजोर, परावर्तक विल मोड़ विथ दोनों हैंड्स. यह a a माइक्रोस्कोप विथ a प्रकाश स्रोत उपयोगित, यह कदम CAN be oMitted, बट a kNoB for एडजस्टिंग THE चमक है आवश्यक है।
4. Install the ssamples. Put the स्लाइड on the stage, और pay अटेंशन कि the side the the Glass Must face up. सुरक्षित the स्लाइड with स्प्रिंग क्लिप्स, और टर्न the knob of the stage mover so कि सामग्री to be प्रेक्षित a aper a aper aper के साथ संरेखित
5. When When focusing, first टर्न the मोटे Focus kNob to लोअर the lens बैरल धीरे, और Observe View THE Seen Se DESCRIPTION, then Observe from the eyePs Eye, और टर्न THE TOeS FOS FOS TOe THE THE SCREEN HAT RELEASE THE SCREEN THE SCREEN SCREEN VIEW PHOTO TAG OFFEc KEB to adjust it back to clear. Operation Note: Do not adjust the फोकस डायरेक्ट अंडर a हाई-मैग्निफिकेशन lens; जब द लेंस बैरल is कम, निरीक्षण द दूरी द लेंस बैरल और द नमूना से द साइड; ज्ञात आलोचन मान आह्य द वस्तु दूरी. यदि u उपयोग a दूरबीन i a the a re1 THE OBSERVER'S Eye, you USE THE USE the Diopter समायोजन सर्कल टू एडजस्ट आईटी. इन एडिशन%2सी द बाइनोकुलार्स कैन बी रिलेटेड टू एडाप्ट टू द दूरी द ऑपरेटर'एस आंखों के बीच।
6. Tप्रेक्षण यदि you उपयोग a मोनोकुलर माइक्रोस्कोप, खुला your आँखें स्वाभाविक रूप से, Observe the Sample with the बाएँ eye, रिकॉर्ड और आरेख दह दाएँ eye, और at the साम समय द फ़ोकस द बाएँ हाथ to make the ऑब्जेक्ट image स्पष्ट और चाल दह नमूना फ़ील्ड of view. दह दाएँ हाथ आँक आकृति और आकृति तक तक संपूर्ण नमूना is प्रेक्षित, so as not to miss or दोहराया. समायोजन of प्रकाश तीव्रता: सामान्य, प्रकाश प्रकाश for दाग वाले नमूने b मजबूत, और प्रकाश रंगहीन या बिना दाग वाले नमूने होना चाहिए b b प्रकाश for कम-शक्ति प्रेक्षण होना b to b to /कम-शक्ति प्रेक्षण होना चाहिए b b कमजोर, और the प्रकाश for उच्च-शक्ति प्रेक्षण दह दह मजबूत. इन अतिरिक्त to सहजिंग दह प्रकाश स्रोत, दह दस्था/समायोजन दह दह दहह दहह आदरण दहहली दहली एपर्चर भी बहुत महत्वपूर्ण है।
(1) अवलोकन विद a निम्न-आवर्धन लेंस कब प्रेक्षण आवरण a कम-शक्ति लेंस मस्ट उपयोग प्रथम, क्योंकि it has a लार्ज फ़ील्ड of view, and it is easy to find the लक्ष्य और निर्धारण दह भाग to be प्रेक्षित करें.
(2) उच्च आवर्धन प्रेक्षण कब मोड़ /कम आवर्धन से उच्च आवर्धन, you only आवश्यकता to थोड़ा-थोड़ा समायोजित करें the ठीक फ़ोकस knob to make the ऑब्जेक्ट image clear. Do not use the मोटे focus knob जब उपयोग a उच्च आवर्धन लेंस, as यह यह आसानी से क्रश the कवरस्लिप और डैमेज द लेंस. कथन कथन दो उद्देश्य लेंस 2c do उपयोग your उंगलियाँ to डायरेक्ट पुश द ऑब्जेक्टिव द ऑब्जेक्टिव लेंस डायरेक्ट द ऑब्जेक्टिव लेंस यह आसानी से कारण the ऑप्टिकल अक्ष of उद्देश्य लेंस to be विक्षेपण, और the स्क्रू थ्रेड of the कनवर्टर b by क्षतिग्रस्त due to असमान बल, और अंत में the converter will be स्क्रैप्ड.
(3) Observation of the oil lens First use the low power lens and the high power lens to move the inspected object to the center of the field of view, and then change the oil lens for observation. Before oil lens observation, the brightness of the microscope should be adjusted to the brightest, and the aperture should be fully opened. When using an oil lens, first drop a drop of cedar oil (lens oil) on the cover glass, then lower the lens barrel and observe carefully from the side until the oil lens is immersed in the cedar oil and is close to the slide specimen, then observe with the eyepiece, and use the fine adjustment The focus knob lifts the lens barrel until the focal length of the specimen is seen clearly and then stops and adjusted clearly. Cedar oil should be added in an appropriate amount. After using the oil lens, be sure to wipe off the cedar oil with a lens cleaning paper dipped in xylene, and then wipe off the excess xylene with a dry lens cleaning paper.
7. Finish the operation. After Observing, remove the नमूना, रिवर्स the कनवर्टर, मेक द लेंस V-आकार to the sides, और द दर्पण be erected. लोअर द लेंस बैरल, वाइप it clean, और put ऑन द लेंस कवर. यदि u are उपयोग a माइक्रोस्कोप विथ a light source, you आवश्यकता to adjust the brightness knob to adasz the brightness to the डार्केस्ट, and then टर्न ऑफ the power button to prevent the instantaneus अत्यधिक धारा से बर्निंग द प्रकाश स्रोत जब u टर्न it on next time.
(2) Inverted Microscope The main difference between an inverted microscope and an upright microscope is that the objective lens is located below the stage, which is conducive to performing some real-time operations on the sample above during observation. The operation process of an inverted microscope is basically similar to that of a binocular upright microscope. The following points should be noted: the hinged binocular eyepieces can be adjusted to a comfortable position during observation. Splashes of tissue culture fluid or water onto the stage, objective, or microscope frame may damage the equipment. If spilled, immediately unplug the power cord from the wall outlet and wipe up the spilled liquid or water. Be sure to turn the light intensity knob gently and do not try to turn the knob past the end position. After use, be sure to adjust the intensity of the light to the minimum before turning off the power. After use, the three-hole converter should be rotated so that the objective lens is placed on the lower side of the stage to prevent dust from settling.
(3) Solid microscope is also called stereo microscope or dissecting microscope. The operation steps are basically similar to those of a binocular upright microscope: when using a dissecting mirror, you need to use both hands to move and keep steady. If the mirror box needs to be moved, the mirror box should be locked, and the key of the mirror box must be removed at the same time. If there is a dust cover on the mirror tube, it should be removed and replaced with the eyepiece and eyecup. Place the sample on a glass slide or in a wax dish and place it on a loading plate for observation. Tighten the screws, raise the mirror body to a certain height first, and then lock the mirror body. The eyepiece tube can be turned to fit the eye distance before observation. The difference in binocular vision can be adjusted by the visual circle. When focusing, do not turn the lifting screw too fast or forcibly, so as not to damage the gear. For magnified observation, turn the magnification dial until the required magnification. The larger the object image, the darker the light, so it is necessary to adjust the light source and select a good background object. After use, first take away the things on the loading tray, and tighten the elastic screws to put down the mirror body and lock it. Remove the eyepiece and replace the dust cover. Put the elements back together, being careful not to interchange with other mirrors. Wipe the mirror body clean with a cloth, put it into the mirror box, and lock the mirror box.
